Journal: Frontiers in Plant Science
Article Title: The mycoparasite Pythium oligandrum induces legume pathogen resistance and shapes rhizosphere microbiota without impacting mutualistic interactions
doi: 10.3389/fpls.2023.1156733
Figure Lengend Snippet: Transcriptomic analysis of Pythium oligandrum -inoculated roots. RNAseq experiments were performed on RNA extracted from in vitro grown plants inoculated with P. oligandrum oospores at different time points. (A) Principal component analysis of data obtained from the RNA sequencing of P. oligandrum and A17 M. truncatula at 3, 5, 7, and 14 days post-inoculation. Orange dots show data belonging to M. truncatula plants inoculated with P. oligandrum , and green dots show data belonging to mock plants. (B) Number of differentially expressed genes (DEGs) at each time point. (C) Venn diagrams representing the DEGs for each condition. (D) Hierarchical clustering on DEGs; clustering was obtained using Hierachical Clustering Explorer version 3.5 with default parameters.
Article Snippet: The sequencing data were processed under R v3.4 ( www.r-project.org ) using the R-Studio ( http://www.rstudio.com/ ) Phyloseq package ( ).
Techniques: In Vitro, RNA Sequencing